AMSBIO reports how researchers at the Washington University School of Medicine (St. Louis, USA) have used their 10E4 Heparan Sulfate (HS) antibody in pioneering obesity research to quantify the role of HS in the process of intercellular mitochondria transfer to macrophages.
In recent published research, researchers from the Brestoff and Teitelbaum Labs demonstrated that adipose-tissue resident macrophages acquire mitochondria from adjacent adipocytes using HS. This process occurs in healthy conditions but is impaired in obesity. Further they have shown that genetic disruption of mitochondria uptake by macrophages reduces energy expenditure and increases diet-induced obesity in mice, indicating that intercellular mitochondria transfer to macrophages mediates systemic metabolic homeostasis.
Obesity is an increasingly common metabolic disease that affects over 40% of adults and 18% of children and adolescents in the United States and is an independent risk factor for the development of many other disorders such as type 2 diabetes, cardiovascular diseases, and cancer.
Dr Wentong Jia, a postdoctoral fellow at the Brestoff lab added “The cell surface expression of heparan sulfate, a glycosaminoglycan required for mitochondria uptake in macrophages, depends on a key glycosyltransferase named EXT1. The 10E4 antibody from AMSBIO has helped us verify that we’ve successfully prevented Heparan Sulfate from being synthesized in cells that lack EXT1.”
AMSBIO offers a comprehensive range of high quality Heparan Sulfate (HS) antibodies from F69-3G10, F58-10E4 and JM403 clones, which are ideal for targeted binding of HS in Heparan Sulfate Proteoglycans (HSPG) research.
Heparan sulfate is a highly sulfated polysaccharide, synthesized as the glycosaminoglycan component of heparan sulfate proteoglycans (HSPGs), that is widely distributed on cell surfaces and basement membranes in mammals. It participates in important biological processes due to it displaying specific interactions with many biologically active proteins. AMSBIO is a leading supplier of high quality Heparan Sulfate antibodies from the important clones (JM403, 10E4 and 3G10), which are ideal tools for investigating the binding of HS in different areas of biological research including infection, cancer, and cell signalling.
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